TISSUE SAMPLE COLLECTION
The following samples will be collected:
3) Bronchial samples including lavage, bronchial brushings and biopsies
4) Nasal lavage
The blood and sputum samples are used immediately or frozen at -80 degrees centigrade and stored. The biopsies are stored in GMA (at -20 degrees centigrade) and paraformaldehyde (at 4 degrees centigrade). Fibroblasts are stored in liquid nitrogen until they are required. The time that these samples can be kept for before they deteriorate is not known, however our previous experience shows that they can all be kept for at least 5 years with no obvious signs of deterioration.
It is anticipated that samples will be collected from approximately 100 volunteers a year. Samples will be sent frozen to other researchers or cooled to the storage temperatures. Approximately 50% of samples will be initially stored. The other 50% will be used immediately. Around 10% of the stored samples will be distributed to third parties.
The following data will be collected and stored for linking with the tissue samples.
Date of birth, age, gender, racial category, vital signs (BP, pulse, respirations), weight, height, BMI, date of diagnosis, family history of asthma, current asthma symptoms and frequency, current asthma medication, other medication, other significant medical/surgical conditions, results of the physical examination (including cardiovascular system, respiratory, ENT, lymph glands, gastrointestinal/genitourinary, neurology), menstrual history, skin allergy test results, spirometry results (included percent predicted values), methacholine challenge results.
The data will be collected by direct contact with the subjects and by performing the respiratory tests and procedures. The medical history will be completed by questioning the subjects. If the details are unclear and the subject agrees their medical notes may be consulted for clarification. This will not be done routinely. For subjects in the COPD group they will be asked to complete a respiratory questionnaire.
The data will be recorded in the case report form and also in the subjects medical notes. The Synairgen team will have access to the case report form which will only contain linked annonymous data. To aid communication some of the data will be stored on a spread sheet on the Synairgen server. This will be annonymous data and will include, age, gender, medications and lung function tests. The classification code and the subject code will be used for points of reference. The data is held on a secure server.